2024 Phic31 integrase system

Phic31 integrase system

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August undefined, 2024

WebTwo orthogonal systems (MS2 and PP7) have been adopted for Drosophila [9-11]. Nascent mRNAs transcribed from the tagged loci carry stem-loops at their 5′ ends, preferably intronic regions. ... We used BDSC #34770 as the maternal phiC31 integrase source. These female flies provide integrase activity in their germlines, which induces attB/attP ... http://www.systembio.com/wp/wp-content/uploads/phiC31_productsheet-1.pdf

Double knock-in pig models with elements of binary Tet-On and phiC31 …

WebThe phiC31 integrase system has, for the first time, enabled transformation of flies with BAC-sized constructs . The integrase can be expressed from mRNA co-injected with the construct or from the genome under a germ-line specific promoter, the latter method being more efficient . Several dozens of ... Web17. mar 2006 · The phiC31 integrase catalyzes precise, unidirectional recombination between its 30-40-bp attP and attB recognition sites. In mammalian cells, the enzyme also … perth interesting places

Site‐Specific Integration with ϕC31 Integrase for ... - ResearchGate

Web4. mar 2016 · Use of phiC31 Integrase for Constructing Transgenic Organisms One application of phage integrases that has been popular is the use of phiC31 integrase to place transgenes into the genome to construct transgenic organisms. Both pseudo att sites and authentic att sites have been used in this regard. Web29. júl 2012 · The phiC31 integrase system is thought to be more efficient than the FLP recombinase for the construction of transgenic insects because it has been successfully applied to diverse organisms, such as humans, flies, and mosquitoes (Groth et al. 2000, 2004; Bateman et al. 2006; Nimmo et al. 2006; Bischof et al. 2007 ). WebPinpoint & PhiC31 Integrase Systems Minicircle Technology Episomal Expression Vectors (EEV) Custom mRNA Synthesis Production Cold Fusion Cloning T Cell Research Tools … stanley martin lowell

Improved site-specific recombinase-based method to produce

Expression of phiC31 integrase in Escherichia coli.

WebA method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly … Web10. apr 2024 · The phiC31 integrase functions efficiently in vitro and in Escherichia coli, yeast, and mammalian cells, mediating unidirectional site-specific recombination between its attB and attP recognition ... perth interior designersWebTo overcome these problems, it is necessary to develop a site-specific integration system. It is known that phiC31 integrase has the capacity to mediate recombination between the target sequences attP and attB. To test the availability of site-specific integration in the silkworm, we first examined the efficiency of recombination between the ... perth international airport flight arrivals

"WebIt is known that phiC31 integrase has the capacity to mediate recombination between the target sequences attP and attB. To test the availability of site-specific integration in the … " - Phic31 integrase system

Phic31 integrase system

WebphiC31 integrase is a site-specific recombinase from a bacteriophage that has become a useful tool in mammalian cells. The enzyme normally performs precise, unidirectional … WebSBI’s PhiC31 Integrase System consists of the PhiC31 Integrase Expression Plasmid and a PhiC31 Donor Plasmid that has your gene-of-interest cloned in. The system enables: Non …

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Web• Developed phiC31 integrase system in Hydra to optimize transgenesis via site-specific recombination. • Designed DNA constructs for microinjections. • Mentored undergraduate students and ... WebThe potential use of the ΦC31 integrase system in gene therapy opens up the possibilities of new treatments for old diseases. ΦC31 integrase mediates the integration of plasmid …

Web25. nov 2024 · Here, we adapted the phiC31 integrase system for C. elegans. We generated an integrated phiC31 integrase expressing strain flanked by attP sites that also serves as a landing pad for... The mode integration/resolution and inversion (INT/RES and INV) depend on the orientation of recombinase target sites (RTS), among these pairs of attP and attB. Section C indicates, in a streamlined fashion, the way recombinase-mediated cassette exchange (RMCE) can be reached by synchronous double-reciprocal crossovers (rather than integration, followed by resolution).

Web18. máj 2024 · Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression. Jin Q1, Yang X1, Gou S1, Liu X1, Zhuang Z1, Liang Y1, Shi H1, Huang J2, Wu H1, Zhao Y1, Ouyang Z1, Zhang Q1, Liu Z1, Chen F1, Ge W1, Xie J1, Li N1, Lai C1, Zhao X1, Wang J1, Lian M1, Li L1, Quan L1, … WebThe phiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage phiC31. The phiC31 integrase mediates recombination between two 34 …

Web23. feb 2010 · Among those, the phiC31 uni-directional recombinase was highly efficient. The system has been successfully shown capable of recombinase mediated excision, inversion and integration reactions. The phiC31- att system is derived from the broad host range Streptomyces temperate phage phiC31 [ 11 ].

WebUnlike the better-known recombinases such as Cre and Flp, PhiC31 integrase catalyzes recombination between two nonidentical sites and lacking a corresponding excision … perth international airport incoming flightsWeb20. mar 2024 · The phiC31 integrase system-based approach was applied to produce transgenic mice via a pronuclear injection, whereby an intact single-copy transgene could … stanley martin homes south carolinaWeb14. sep 2012 · The Streptomyces phage phiC31 integrase was tested for its feasibility in excising transgenes from the barley genome through site-specific recombination. We produced transgenic barley plants expressing an active phiC31 integrase and crossed them with transgenic barley plants carrying a target locus for recombination. The target … perth international airport arrivalsWeb28. feb 2014 · PhiC31 integrase-mediated gene delivery has been extensively used in gene therapy and animal transgenesis. However, random integration events are observed in … stanley martin lowell modelWeb18. máj 2024 · Use of phage PhiC31 integrase as a tool for zebrafish genome manipulation. In: Detrich, H.W., Westerfield, M., and Zon, L.I., eds. Methods in Cell Biology. Zebrafish: Genetics, Genomics and Informatics, 3rd Ed. New … stanley martin marlboro ridingWeb17. sep 2008 · The phiC31 integrase system is expected to be functional in other monocotyledonous species and in commercial wheat varieties. We succeeded in transforming phiC31 integrase vectors into rice ( Oryza sativa ), pearl millet ( Pennisetum glaucum ) and varieties of commercial winter wheat provided by the Nordsaat Saatzucht … perth international airport flight trackerWebBackground: PhiC31 integrase system provides a new platform in various felid of research, mainly in gene therapy and creation of transgenic animals. This system enables integration of exogenous... perth international arrivals today