Human monocytic cell line thp-1
WebTo foster research on the ASC adaptor, InvivoGen provides cellular tools that have been generated from the human monocytic THP1 cell line. THP-1 cells are widely used for … WebLysophosphatidylcholine induces platelet-derived growth factor gene expression in a human mesangial cell line. Top. Search. Home > Section 11 > Chapter 10,946. Lysophosphatidylcholine induces platelet-derived growth factor ... The mRNA expression level in HMCL treated with 50 muM lysoPC for two hours increased to 1.6-fold that of the …
Human monocytic cell line thp-1
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WebDifferentiation of THP-1 monocytes to macrophages. To initiate the differentiation of monocyte-derived macrophages, 30 ng/mL of phorbol-12-myristate-13-acetate (PMA, Sigma-Aldrich) was added to the RPMI-1640 medium (10% FCS, 1% PS) for 3 days, where human monocytic leukemia cells (THP-1) were grown in suspension. Web18 nov. 2016 · THP-1 designates a spontaneously immortalized monocyte-like cell line, derived from the peripheral blood of a childhood case of acute monocytic leukemia (M5 …
WebTHP-1 cells are a monocyte-like cell line derived from a patient with acute monocytic leukemia and unlike other leukemic cell lines has a normal diploid karyotype. We … Web7 nov. 2016 · Compounds 1, 2, 3, and 4 showed significant selective cytotoxicity against human acute monocytic leukemia cell line (THP-1) with IC50 values of 5.12 μM, 1.78 μM, 1.74 μM, and 1.75 μΜ, respectively. Compound 1 also showed an anti-inflammatory effect through the inhibition of the activity of NF-κB by blocking the nuclear translocation of p65.
WebMonociles and their macrophage and dendritic-cell progeny serve three main functions the the immune system. Those have phagocytosis, antigen showcase, and cytokine production. THP-1 and U937 are (pro-) monocytic cell lines that can, also in vitro, be differentiated into either various types of microscopic or into dendritic cells. Web1 dec. 2024 · Macrophages are the preferential cell types to study various aspects of mycobacterial infection. Commonly used infection models for in-vitro studies are primary …
WebTHP-1 is a monocytic cell line engineered for monitoring NF-κB activation by determining the activity of secreted embryonic alkaline phosphatase. The growth medium consisted of RPMI-1640 supplemented with 2 mM L-glutamine, 25 mM HEPES, 10% (v/v) heat-inactivated FBS, 100 μg/mL normocin, and 100 U/mL penicillin-100 μg/mL streptomycin. topi gordon reeceWeb1 okt. 2000 · THP-1 is a human monocytic cell line, 9 which matures into macrophage-like adherent cells following stimulation with phorbol 12-myristate 13-acetate 10 or 1α, 25 … topi fashionWeb30 apr. 2024 · Human aortic endothelial cells (HAECs) were obtained from Lonza (Basel, Switzerland) and the human monocytic leukemia THP-1 cell line was purchased from ATCC (USA). HAECs were maintained using a Microvascular Endothelial Cell Growth Medium-2 BulletKit from Lonza, USA. THP-1 cells were cultured in DMEM media … topi class 8 question answerWebIn this study, we characterized a set of ovarian cancer-specific T-cell epitopes delivered by live-attenuated recombinant Listeria monocytogenes (Lm DeltaactADeltainlB) as a vaccine vector. We present data that peptide-specific T cells recognize the human monocytic cell line THP-1 infected with recombinant Lm DeltaactADeltainlB encoding the... topi highlightWebAbstract: We investigated TLR10 expression in human monocytes, THP-1 cells, cultured in hypoxia (3% O 2). Levels of both TLR10 mRNA and protein in THP-1 cells cultured in hypoxia were significantly higher than those cultured in normoxia (20% O 2). We examined intracellular reactive oxygen species (ROS) content in hypoxic cells, and TLR10 topi freesmachineWebHerein, we describe the design of a library of their analogues and the evaluation of their apoptosis-inducing activities against the human … topi fintechWebTHP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and … topi folding cooler