2024 Hct116 p53+/+

Hct116 p53+/+

Author: wzrq

August undefined, 2024

WebAug 25, 2009 · The use of p53-specific siRNA in HCT116 (p53 +/+) cells also elicited the same divergent pattern of p53 transcriptional behavior, where Lasp1 mRNA was up … WebEnter the email address you signed up with and we'll email you a reset link.

Gene expression profiles in HCT116 and HT29 cells exposed to …

WebKSHV; ORF50; MDM2; Sp1; p53; transcriptional repression 1. Introduction Kaposi’s sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus 8 (HHV8), is implicated as the causative agent of several human malignancies, including Kaposi’s sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman’s disease [ … WebApr 7, 2024 · E-cadherin and vimentin staining of metastatic tumors in the livers ( G) and lungs ( H) with HCT116-sh-DDX21 and HCT116-sh-NC cells. Scale bar stands for 50 μm length. The protein expression... delete temporary files and browser cache

Regulation of p53 Stability and Function in HCT116 Colon …

WebHCT116 cells treated with ES (100 µg/mL) were starved in 0.2% FBS and were serum-stimulated (with 20% FBS). Afterward, when the expression of c-Myc was confirmed, it was found that the ES was regulated. 2.5. ES Reduces c-Myc Protein Stability in Colon Cancer Cells We evaluated the stability of c-Myc using CHX. WebDec 2, 2005 · p53 is the most frequently mutated gene in human cancer (Greenblatt et al, 1994). It exerts tumour-suppressor activity, regulating the cell cycle, programmed cell death and DNA repair. p53 functions are mediated by mechanisms that are transcriptional (Yu et al, 1999) and non-transcriptional (Mihara et al, 2003) dependent. HCT116 cells have a mutation in codon 13 of the KRAS proto-oncogene, and are suitable transfection targets for gene therapy research. The cells have an epithelial morphology and can metastasize in xenograft models. When transducted with viral vectors carrying the p53 gene, HCT116 cells remain arrested in the G1 phase. The proliferation of HCT116 colonies was found to be inhibited by 5-Fu/P85 copolymer micelles. Furthermore, it was found that the knockout of MARCH2 limited … delete temporary files edge

Characterization of p53 Wild-Type and Null Isogenic Colorectal …

Redefining the p53 response element PNAS

WebApr 13, 2024 · HCT116, HCT116 p53 −/−, and HCT116-5FUR cells were treated with mannose (50 mM) and 5-FU (5 μM) alone or in combination for up to 72 h. Cells treated … WebMay 1, 2007 · We have previously reported that sensitive wt p53 cancer cell lines such as colon HCT116 and RKO or hepatoma HepG2 are killed through p53-mediated apoptosis. … ferieplan 2023 simployer ferie officiel a geneve

"WebMar 24, 2024 · PGA 2 may induce p53-dependent apoptosis in which DNA-PK activates p53, and DR5, a transcriptional target of p53, plays a pivotal role in HCT116 cells. In contrast to apoptosis in HCT116 cells, PGA may induce apoptosis in a fashion of less potency, which is independent of p53 and DNA-PK in HCT116 p53-/- cells. " - Hct116 p53+/+

Hct116 p53+/+

JNK confers 5-fluorouracil resistance in p53-deficient and mutant p53 …

WebApr 13, 2024 · HCT116 p53 −/− cells were cultured in a DMEM medium (Lonza) supplemented with 10% FBS, 100 U/mL of penicillin-streptomycin, 1 mM of sodium pyruvate, and 1× MEM non-essential amino acid (Sigma-Aldrich). All cells were incubated in a humidified incubator (95% air, 5% CO 2) at 37 °C. 2.3. Drugs and Compounds WebATR and ETV1 promote TERT expression in human cancer cell lines expressing a p53 mutant and in HeLa cells. (A) Immunoblot analysis monitoring TERT levels in various …

Did you know?

WebMar 24, 2004 · The p53 gene was inactivated in HCT116 p53 −/− cells by homologous recombination. Briefly, two promoterless targeting vectors containing either a geneticin or hygromycin resistance gene in place of genomic p53 sequences were sequentially transfected into HCT116 p53 +/+ cells to disrupt both p53 alleles (12). WebTherefore, HCT116 p53+/+ cells are simply marked as HCT116 except for in Figure 1. Our results confirmed that ES induced apoptosis by reducing the expression of c-Myc and …

WebMay 21, 2024 · These included HCT116, HCT116.p53 mutant, RKO, and RKO.p53 -/- lines (all from colon cancers) as well as breast cancer cell lines MCF7 and 1001 (MCF7-p53 mutant clone). HeLa cell line was used as a positive control for epithelial to mesenchymal transition (EMT). WebNov 30, 2024 · HCT116, HCT116-p53−/−, LS513, LS1034, and DLD-1 were cultured in RPMI. For LIM1215 cells, RPMI was supplemented with 0.6 µg/ml human insulin (Sigma). For MDST8, HT-29, LoVo, and GP5D, DMEM was...

WebTo demonstrate that the p53-Luc fusion retains p53 function, we utilized HCT116 p53–/– cells (kindly provided by Dr B. Vogelstein). The pZ p53-Luc expression plasmid was stably transfected into HCT116 p53–/– cells and the resulting clones were used for … WebAug 8, 2014 · We verified by PCR however, the HCT116 p53-/- still express all the p53 isoform (D40p53alpha,beta,gamma), d133p53alpha,beta,gamma and also the …

WebIn summary, p53-null HCT116 cells transduced with a lentiviral vector expressing wild type p53 respond to DNA-damaging and …

WebJan 17, 2006 · This analysis was based on a combination of denaturing HPLC mutation screening of all exons of the p53 gene, sequencing the cDNA, and assessing the … delete temporary files and cookies in edgeWebSep 16, 2024 · Cell proliferation of A549 and HCT116 cells was evaluated using the [4,5-dimethylthiazol-2-yl]-2,5-di-phenyltrazolium bromide (MTT) assay. Briefly, cells were seeded in a 24-well plate and incubated overnight. At the indicated time points, MTT solution was added to a final concentration of 1 mg/mL and incubated for 3 h. delete temporary download filesWebAug 25, 2009 · The use of p53-specific siRNA in HCT116 (p53 +/+) cells also elicited the same divergent pattern of p53 transcriptional behavior, where Lasp1 mRNA was up-regulated to 154%, whereas p21 mRNA was repressed by p53 siRNA by 63% relative to that of control ( P < 0.05; Fig. 1 B ). Fig. 1. p53 represses Lasp1 transcription. delete temporary files on iphoneWebMay 19, 2016 · SIRT1 regulates a wide variety of cellular processes, particularly longevity, metabolic control, genotoxic responses, and autophagy, through deacetylating transcriptional factors p53, Forkhead box protein O1 (FOXO1), FOXO3a, NF-κB, MYC, E2F1 and Hypoxia-Inducible Factor (HIF)-1α/HIF-2α, and DNA repair proteins [ 39 ]. ferie professoriWebJul 20, 2024 · Comparable radiosensitivities of p53 +/+ and p53-/-HCT116 colorectal cells induced by CIR were demonstrated, as well as persistent 53BP1 foci indicated DNA repair deficiency in both cell lines. Different degree of premature senescence in isogenic HCT116 colorectal cancer cells suggested that CIR-induced premature senescence was more … ferier cateringWebFeb 9, 2024 · To test this therapy model in vivo, we established HCT116 xenograft tumors and compared p53 levels in response to ionizing radiation alone or in combination with … ferier cornetWebMar 15, 2004 · To elucidate mechanisms of resistance to chemotherapies currently used in the first-line treatment of advanced colorectal cancer, we have developed a panel of HCT116 p53 wild-type (p53 (+/+)) and null (p53 (-/-)) isogenic colorectal cancer cell lines resistant to the antimetabolite 5-fluorouracil (5-FU), topoisomerase I inhibitor irinotecan … delete terminated instance oracle cloud