Hct116 mitotic slippage
WebMar 16, 2024 · The mitotic indices of HCT116 cells with and without APC-ΔC expression treated with 100 nM BI6727 peaked at 20–24 h. ... Previous studies indicated that mitotic slippage occurs via slow Cyclin ... WebFigure 1.activation of apC substrate degradation during mitotic slippage of synchronized hCT116 cells. (a) Cell cycle profile of hCT116 cells synchro- nized at G 1 /s with thymidine (Thy), released and then treated with nocodazole (Noc, 500 ng/ml, 1.66 µM) at 1 hour after release from thymidine.
Hct116 mitotic slippage
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WebMar 1, 2024 · Mitotic slippage was also observed with even higher frequency in JH-Eso-Ad1 cells (28.6%; n = 7) ... (HCT116), colorectal adenocarcinoma (HT29), osteosarcoma (U2OS) and cervix adenocarcinoma (HeLa) cells [34, 35]. These findings led to speculate that increased accumulation of Ndc80, as part of the Ndc80 complex, might influence the … WebUsing cells blocked in mitosis with the microtubule assembly inhibitor nocodazole, we show that mitotic APC substrates are degraded upon mitotic slippage. To confirm that APC is normally activated upon mitotic slippage we have found that knockdown of Cdc20 and Cdh1, two mitotic activators of APC, prevents the degradation of APC substrates ...
Web(a) Most HCT116 cells escape from mitosis at 24 h after paclitaxel treatment. (b) In contrast, almost 40% of HT29 cells are still in mitosis after 48 h of paclitaxel treatment. The mitotic... WebWe previously profiled the anti-mitotic drug response of 11 mammalian cell lines, including cancer cell lines derived from the lung (A549, H460), colon (HCT116, HT29), breast (MCF7, MDAMB435S), ovary (OVCAR5), cervix (HeLa), prostate (PC3), and bone (U-2 OS) as well as a normal cell line, RPE.5
Weblowed by mitotic slippage is required for the activation of Bax, et al., 1999; Kapoor et al., 2000). KSP-IA induced the formation ... Using a recombinant KSP motor domain, colon cancer cell line HCT116. Thus, KSP-IA is a potent, spe-a high-throughput screen was employed to identify inhibitors of cific, and cell-active inhibitor of KSP, and it ... Given that p53 is activated by different Aurora inhibitors, we next examined if the mitotic responses to the Aurora inhibitors are influenced by p53. Clones of HCT116 and HCT116(p53−/−) stably expressing histone H2B-GFP were generated. The cells were examined with time-lapse microscopy after challenged with … See more To examine the relationship between p53 and inhibition of AURKA and AURKB, several p53-containing cell lines were challenged with different Aurora inhibitors. When HCT116 cells were incubated with Alisertib, both p53 … See more In addition to inducing mitotic slippage, Alisertib also delayed mitotic exit in p53-deficient cells (Figure 4a), suggesting that AURKA inactivation … See more
WebMay 19, 2016 · To determine whether SIRT2 is involved in SAC functions, we used the colorectal cancer cell line HCT116 (a mitotic checkpoint-proficient near-diploid cell line), …
gunwerks complaintsWebMay 19, 2016 · Microtubule poisons inhibit spindle function, leading to activation of spindle assembly checkpoint (SAC) and mitotic arrest. Cell death occurring in prolonged mitosis is the first target of microtubule poisons in cancer therapies. However, even in the presence of microtubule poisons, SAC and mitotic arrest are not permanent, and the surviving cells … boxes for mini cakesWebApr 30, 2009 · C, detection of phosphorylated Aurora-A and Aurora-B kinases on Western blots using lysates from mitotic HCT116 cells treated with nocodazole for 14 h followed by incubation with MG132 in the presence or absence of 2 μmol/L Gö6976 or 2 μmol ... Mitotic checkpoint slippage in humans occurs via cyclin B destruction in the presence of an … boxes for mailing wineWebJul 1, 2005 · Three lines of evidence indicate the dependence of apoptosis on mitotic slippage in checkpoint-intact cells: (1) in both HCT116 and A2780 cells, KSP inhibitor … boxes for mailing travel mugsWebNov 28, 2024 · We found that the mitotic slippage did not lead to additional DNA damage, as A9 cells treated with colchicine for 48 hr showed similarly low frequency of comets as untreated cells. ... (referred to as RPE-1) and RPE-1 hTERT H2B-GFP were a kind gift of Stephen Taylor (University of Manchester, UK). HCT116 H2B-GFP was generated by … gunwerks co pilotWebNational Center for Biotechnology Information gunwerks.comWebcally reduced by 24 hours in HCT116 cells, implying that mitotic slippage is accompanied by the activation of APCCdc20 (Fig. 1E, Suppl. Fig. S2). Cdh1, another APC activator, … gunwerks company