WebJan 1, 2024 · Using cell surface biotinylation of live cells and receptor pull-down assays, we compared the receptors internalized in control untreated cells and those internalized upon exposure to nanoparticles. In this way, we identified receptors associated with (high) nanoparticle uptake. ... Isolation of cell surface proteins for mass spectrometry-based ... WebThe Thermo Scientific Cell Surface Protein Isolation Kit is a complete set of indicators for selective biotinylation press subsequent purification of mammalian cell surface …
Cell surface protein biotinylation for SDS-PAGE analysis
WebMar 22, 2024 · Cell surface biotinylation. Plasma membrane proteins of HEK293T cells were surface biotinylated by incubation of ~ 1 × 10 7 cells/ml in membrane-impermeable biotinylation reagent Sulfo-NHS-Biotin (1 mg/ml, in HBSS; Abcam, Cambridge, UK) for 30 min following the manufacturer’s protocol. After that, free biotin was quenched by … WebMar 8, 2024 · The cell surface proteome, termed the surfaceome, serves as the main communication hub between a cell and the extracellular environment (Wollscheid et al., 2009).As such, this cellular compartment often reveals the first signs of cellular distress and disease, and is of substantial interest to the medical community for diagnostic and … scotsman n0622a-1e
Cell surface protein biotinylation for SDS-PAGE analysis
WebMar 16, 2024 · Abstract. Cell surface is the primary site for sensing extracellular stimuli. The knowledge of the transient changes on the surfaceome upon a perturbation is very important as the initial changed proteins could be driving molecules for some phenotype. In this study, we report a fast cell surface labeling strategy based on peroxidase-mediated ... WebOne of the compartments of substantial interest from a diagnostic and therapeutic point of view is the plasma membrane which contains intrinsic membrane proteins and other proteins expressed on the cell surface. Technologies are currently available for the comprehensive profiling of the cell surface proteome that rely on protein tagging of ... WebMar 5, 2024 · With a labeling time of 1 min, 2684 proteins, including 1370 (51%) cell surface-annotated proteins (cell surface/plasma membrane/extracellular), 732 trans-membrane proteins, and 81 cluster of ... scotsman n0422a-1a