WebCell Counting Kit-8は細胞増殖試験、細胞毒性試験、化学物質の感受性試験等において、細胞数を測定するキットです。 高感度水溶性ホルマザンを生成する新規テトラゾリウム塩WST-8を発色基質として採用することで、従来のCell Counting Kitより高感度測定が可 … WebStandard curves of Cell Counting Kit-8 (CCK-8; Dojindo Laboratories, Kumamoto, Japan) for HaCaT cell proliferation according to the frequency of passage in the 5% PL and 5% FBS groups. Optical...
CCK8 - protocols and tips
WebDojindo is the original creator of WSTs dyes and the Cell Counting Kit-8 that are used all over the world. Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. Dojindo's highly water-soluble tetrazolium salt (WST-8) is reduced by dehydrogenase ... WebSep 17, 2024 · Cell proliferation detection by CCK8 analysis. K562 cells transfected with HBA1 and HBB1 showed slow-growing curves compared to the control. Display full size Overexpression of HBA1 and HBB promoted apoptosis in K562 cells To quantify the effects of HBA1 and HBB on apoptosis in K562 cells, Annexin-V and PI staining assays were … asturi malaysia
同仁化学/DOJINDO LABORATORIES
Webproliferation and cytotoxicity assays. Dojindo’s highly water-soluble tetrazolium salt, Wst-8, is reduced by dehydrogenase activities in cells to give an orange-colored formazan dye, it is soluble in the tissue culture media. The detection sensitivity of K-8 is higher than that of other tetrazolium salts such as MTT, XTT or MTS. PRIN IPLE WebCell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. Dojindo’s highly water-soluble tetrazolium salt, Wst-8, is reduced by dehydrogenase activities in cells to give an orange-colored formazan dye, it is soluble in the tissue culture media. WebFeb 23, 2024 · The Cell Counting kit-8 (CCK-8) assay (CK04-500; Dojindo, Japan) was used for the cell viability test. BV2 cells were cultured in 96-well plates until 70% confluence was reached. The cells were then treated as described above. Different concentrations of artesunate were added for another 24 h. Next, 10 μl of CCK-8 working solution was used … a sub dark transfer paper